Proxcys B.V. is the world's primary producer of high performance low pressure cGMP compliant radial chromatography equipment

Micro RFC

Micro RFC columns: chromatographic performance of the radial column format in analytical scale volumes. Since the Micro RFC columns are an accurate scale-down...

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Lab HP-RFC

Lab HP-RFC columns offer “Radical” Chromatographic performance on bench scale. Ultra fast processing in robust processes with any resin and easy handling. Lab columns' suitability...
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SUPR columns

Extended range Micro to Process RFC columns in a prepacked-prevalidated format "plug-and-produce". Prepacked columns can be used for extended periods, dedicated projects...
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Pilot Process Industrial RFC

Proxcys High-Performance Radial Flow Chromatography (HP-RFC) columns are applicable from Pilot to Industrial scale operations. The "work-horses"...

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Accessories & Systems

Proxcys offers a full range of cGMP compliant accessories including sanitary Bubble traps, Intelligent packing systems and miscellaneous...
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Custom Engineering

Proxcys will develop and supply ancillary equipment that support your Downstream Processing tasks and handling. Sophisticated 3D modelling will allow fast turnaround...
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Principle of Operation

The Annular packing of the HP-RFC columns results in a uniform packed bed. In the packed bed the resistance of the resin is the only parameter to determine ΔP over the length (height) of the bed.Principle_of_operation2

 

The bed length (height) is identical in A-B-C-D-E. Hence the resistance by the resin is identical in A-B-C-D-E, thus the ΔP is identical. Absolute P (top to bottom) is influenced by the height of the the column. However the water column exists on outer and inner space (communicating vessels) and therefore ΔP is still only determined by the resistance of the resin.

 

The dead-space outside the outer frit and inside the inner frit are similar the vertical liquid velocity in the dead-spaces “Vo” and “Vi” are identical Vo=V. The sloped line is a dramatized representation of the product-front. It indicates the saturation of the column will start from the top down. The "head-start" that the product in "lane-A" has over "lane-E" is lost again on the inside of the column since Vo=Vand the vertical speed is identical. Hence the height (= volume !) of the column does not influence the performance of the radial column.

 

Why would the liquid not take a diagonal through the column? The diagonal is a longer distance through the bed = more resistance. Since liquid will always take the path of least resistance. All flow will automatically align horizontal.

 

(A=B=C=D=E)

 

  • Identical Pressure drop

(ΔP1 = ΔP2 = ΔP3)

 

  • Identical velocity

(Vo = Vi)   (RV0 = RVI)

 

Suitability of radial columns for compressible media is explained in fig. 2 and 3. The forces on the media are partially radial and partially tangential causing less deformation and therefore resulting in less resistance = less operating pressure = higher flow. Even Metacrylate and polystyrene are compressed. Apart from the obvious suitability of agaroses sepharoses and celluloses, polymeric resins are extremely well suited for radial columns.

 

Pressure_and_bed_stability_axial

 

      

Pressure_and_bed_stability_radial

 

 

Fig 2: Radial Forces

  

Fig 3: Axial Forces

 

Radial:

Normal (radial) forces partially absorbed tangentially (sideways)

  • Reduced directional compression
  • Reduced deformation (soft gel)
  • Reduced flow resistance

 

Axial:

Compression forces are in-line to the direction of the flow

  • Increased directional compression
  • Increased deformation (soft gel)
  • increased flow resistance

Official Distributor of QUATTROFLOW

 

Proxcys is official QUATTROFLOW distributor for the BeNeLux. Quattroflow pumps, made in Germany (part of the globally operating PSG), are renowned for their outstanding performance, low shear, stability and low-pulse operation.

Proxcys B.V. is the world's primary producer of high performance low pressure cGMP compliant radial chromatography equipment.

Contact

Proxcys BV

Bedrijvenweg 4

NL-7833 JH Nieuw-Amsterdam

The Netherlands

Phone: +31 591 677 447

Fax: +31 591 677 448

Email: info@proxcys.com